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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Mol Cancer Res. 2013 Feb 5;11(5):494–505. doi: 10.1158/1541-7786.MCR-12-0528

Figure 4. ChIP reveals the presence of phosphorylated p65 and RNA Pol II at the promoters of IL-6, IL-8 and SOCS3 of JSI-124 treated cells.

Figure 4

U251-MG cells were left untreated (UT) or treated with JSI-124 (1 μM) or DMSO (vehicle) for 1 h. Immunoprecipitation was performed with 5 μg of Ab to phospho-p65 (A), total p65 (B), phospho-RNA Pol II (C) and STAT3 (D). The immune complexes were absorbed with protein A beads or protein G beads blocked with bovine serum albumin and salmon sperm DNA. Immunoprecipitated DNA was then subjected to semi-quantitative PCR and analyzed by gel electrophoresis for IL-6, IL-8 and SOCS3. The experiment was repeated, and similar results were observed.