Fig. 3.
TFPIβ blocks CHO-TF cell migration through Matrigel by preventing thrombin generation and activation of PAR-1. Cellular migration was standardized to that of CHO-TF cells. (A) Migration of CHO-TF/TFPIβ cells compared to CHO cells, CHO-TF cells and CHO-TF cells in the presence of 100nM/well recombinant TFPI-160, glycosylated TFPIα or non-glycosylated TFPIα (mean ± SEM, n=3). (B) Migration of CHO-TF cells in the presence of SCH79797 (PAR-1 antagonist), rNAP-C2 (TF-FVIIa inhibitor), rTAP (FXa inhibitor), or argatroban (thrombin inhibitor), all at 10 μM (*p<0.001, †p<0.01) (mean ± SEM, n=3).