Figure 1. Suppression of pathogen-induced gene expression in OsLYP4- and OsLYP6-RNAi rice plants. (A) The fourth leafs at the five-leaf stage from each rice line were inoculated with M. oryzae (10⁶ colony-forming units/mL) and the induction fold of Beta-Glu or PAL gene was examined by qPCR at 0, 12, 24 and 36 h after inoculation. (B) The induction fold of Beta-Glu or PAL gene was examined by qPCR at 0, 6, 12 and 24 h after X. oryzaecola (10⁶ colony-forming units/mL) inoculation. The induction fold of each gene was calculated by the gene expression level in pathogen–treated seedlings relative to that in mock-treated seedlings at the same time point. hpi, hour post inoculation. Three biological repeats were conducted, and the data represent means ± SD (10 leaves in each transgenic line, three independent experiments). WT: wild type, CK: control transgenic rice transformed with empty vector, OsLYP4-RNAi-line -1, OsLYP4-RNAi -2, OsLYP6-RNAi line -2 and OsLYP6-RNAi line -5 were compared.