Figure 2. OsLYP4 and OsLYP6 Can Interact with other LYPs. (A) Yeast two-hybrid analysis of the protein-protein interaction between OsLYP4, and OsLYP6. Yeast cells harboring the indicated bait (cloned into the pGADT7) and prey plasmids (cloned into the pGBKT7) were diluted by 1:1, 1:10 or 1:100 and spotted on the selective medium SD-LW (medium without leucine and tryptophan) and SD-LWHA (medium without leucine, tryptophan, histidine and adenine). Growth on the SD-LWHA medium indicates a positive interaction. (B) Interactions between OsLYP4 and CEBiP proteins in yeast and rice protoplasts. BiFC (bimolecular fluorescence complementation) indicated the complementary yellow fluorescence, Chl- indicated the chloroplast autofluorescence in the protoplast. Bar = 10 μm.