Fig. 4.

ELISA of peptides selected against lysozyme both on solid-phase and in-solution selection. After five rounds of selection, peptides expressed on phage as M13 gpVIII fusions were checked for specificity of binding to lysozyme (50 μg/ml), ovalbumin (50 μg/ml), Bcl-X (10 μg/ml), BSA (200 μg/ml), and anti-V5 antibody (10 μg/ml). Two examples of the selected clones are shown; E6 and F11, from solid-phase and in-solution selection, respectively. The V5 peptide fused to gpVIII was used as a both a negative control (against lysozyme) and a positive control (against anti-V5 antibody) for the assay.