Figure 1. Effect of low temperature stress on Arabidopsis Atcyp710a1 mutant and AtCYP710A1 overexpressors. Arabidopsis wild-type (Col-0), Atcyp710a1 mutant and AtCYP710A1 (OE 6–2 and OE 5–1) overexpressor plants (5 weeks old) were exposed to gradual acclimation stress by reducing the temperature of the growth chamber from 20°C to 7°C over a period of two days and then subjected to low temperature stress (7 ± 1°C) for 30 d. At the end of the stress period, photographs of control plants grown under normal growth conditions (A) and low-temperature-stressed plants (B) were taken. Shoot biomass gained over a period of 33 d of stress was measured (C). Further, membrane leakage (D) and total chlorophyll content at the end of the stress were estimated and expressed as a reduction over no stress controls (E). ANOVA was performed (p = 0.05), and letters above each bar indicate the significance and bars with the same letters are not significantly different. Error bars indicate standard error values for three replicate values. For (E), asterisks over the bars represent statistical significance (student t-test) over corresponding Col-0 values.