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Figure 2. ELISA assay using the anti-MLG monoclonal antibody detects abundant MLG in mature stem tissues of several grasses. Senesced stems were ground to a fine powder using a bead beater and treated with 4 M KOH to extract cell wall polysaccharides. The 4 M KOH extracts were used in the ELISA assay, as described before.26 Senesced stems from Arabidopsis plants were used as negative control.
