Table 1.

Molar ratios used for making MPAD-protein (M1–M5) and 2,4-D-protein conjugates (D1–D5)

Protein concentration, μmol (10 mg)	Hapten concentration	Protein–hapten ratio
0.15	0.75 (15 μl)	1:5 (M1 and D1)
0.15	1.50 (30 μl)	1:10 (M2 and D2)
0.15	3.00 (60 μl)	1:20 (M3 and D3)
0.15	6.00 (120 μl)	1:40 (M4 and D4)
0.15	15.0 (300 μl)	1:100 (M5 and D5)