Fig. 5.

Concentration-dependent effects of CGPs on MRP2-mediated uptake of [³H]E₂17βG into inside-out membrane vesicles. (A) Immunoblot of membrane vesicle proteins prepared from MRP2-transfected and untransfected HEK293T (HEK) cells is shown. MAb M₂I-4 was used to detect MRP2. (B–F) Effect of CGPs on MRP2-mediated [³H]E₂17βG uptake activity. Results shown are representative concentration response curves. Data points represent means of duplicate determinations. (B) CGP I-5; (C) CGP III-1; (D) CGP V-3; (E) CGP V-4; and (F) CGP V-6. Similar results were obtained in at least two additional independent experiments. Means (± S.D.) can be found in the text.