Fig. 2.

Brain endocannabinoid, arachidonic acid, and fatty acid derivative levels are differentially affected by acute versus repeated administration and low versus high doses of JZL184. Acute and repeated low- (4 mg/kg) and high-dose (40 mg/kg) JZL184 significantly increased brain levels of 2-AG (A) and decreased brain AA levels (B). (C–E) Repeated administration of high-dose JZL184 increased levels of AEA, PEA, and OEA, whereas acute high-dose as well as acute or repeated low-dose JZL184 had no effect. Data presented as the mean ± S.E.M. (n = 6). *P < 0.05; **P < 0.01 versus vehicle.