Fig. 1.

Protein-protein interaction between AhR and KLF6. C57BL/6 mice were gavaged with either vehicle (-) or 20 µg/kg TCDD (+) 2 hours before sacrifice and preparation of liver nuclear extracts as described in the Materials and Methods section. (A) Nuclear proteins were immunoprecipitated with an antibody against KLF6 and immunoblotted to detect the presence of KLF6 and the AhR. (B) Human recombinant ³⁵S-labeled KLF6 and unlabeled AhR and Arnt were synthesized using the in vitro transcription and translation system. KLF6 was incubated with either the AhR or Arnt protein in the absence (-) or presence (+) of 6 nM TCDD for 2 hours prior to coimmunoprecipitation with antibodies directed against the AhR or Arnt protein. Coimmunoprecipitated KLF6 was detected by autoradiography after fractionation by SDS-PAGE. IP, immunoprecipitation.