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Effect of compound 2 on the growth of mycobacterial species using a spot culture growth inhibition assay. Approximately 500 Mycobacterium bovis BCG and Mycobacterium tuberculosis H37Rv cells were spotted on solidified Middlebrook 7H10 agar containing different concentrations of compound 2 in a six-well plate. Pictures were taken using a digital camera after 14 days of inoculation.
