Figure 3.

Mig6 Interacts with and Activates c-Abl

(A) c-Abl or Mig6 were immunopurified from HEK293T cells ectopically coexpressing Mig6 and wild-type (WT), catalytically inactive (KD), or constitutively active (PP) forms of c-Abl and examined by immunoblotting as indicated.

(B and C) Immunopurification of endogenous Mig6 from pMECs cultured in the presence or absence of EGF for 2 hr and examined for interaction with c-Abl (B) or EGFR (C).

(D) Immunoprecipitated EGFR from HEK293T cells ectopically coexpressing EGFR and Mig6 or mutant forms of Mig6 lacking the AH1 domain (deletion aa 328–361) or the Grb2 binding site (PP319/20AA) and examined by immunoblotting as indicated.

(E) Immunoprecipitation of c-Abl coexpressed with Mig6 and EGFR in HEK293T cells followed by immunoblotting as indicated.