Fig. 2.
GluN3 subunits are incorporated into the NMDA receptors. A, Effect of subunit composition on steady-state current. Bars represent mean current (error bars are 95% CI, n = 40–85). Significant differences compared to GluN1-1a/2A within a voltage group are indicated above the bars (*P < 0.05 or ***P < 0.001, Bonferroni). B, Glycine does not activate a current in oocytes injected with GluN1-1a, GluN2A and GluN3A. Currents from the same oocyte exposed to glycine alone (1 mM) or NMDA/glycine (100/10 μM). Similar results were obtained from six oocytes from three different batches (Vh = − 50 mV). C,D, I/V relationships for oocytes injected with GluN1-1a/2A (C) or GluN1-1a/2A/3A (D). Current was evoked by application of 100 μM NMDA plus 10 μM glycine, normalised to that at − 75 mV and plotted as mean ± S.E.M. Points between − 50 mV and 0 mV are fit by linear regression.