Fig. 4.

Transcriptional consequences of lignin and xylan down-regulation on (A) lignin biosynthesis (B) cell wall polysaccharide biosynthesis and (C) vascular transcription factors in lines asccr, sc4h, asprx and asuxs. qRT-PCR was performed for xylem-specific transcripts (A) lignin [PHENYLALANINE AMMONIA LYASE (PAL), CINNAMATE 4-HYDROXYLASE (C4H), COUMAROYL-ESTER-3-HYDROXYLASE (C3H), CAFFEIC ACID O-METHYL-TRANSFERASE (COMT), CAFFEOYL-COA O-METHYLTRANSFERASE (COCOMT), CINNAMOYL-COA REDUCTASE (CCR), CINNAMYL ALCOHOL DEHYDROGENASE (CAD)], (B) cellulose [CELLULOSE SYNTHASE (CESA), SUCROSE SYNTHASE (SUSY)], and xylan [UDP-GLUCOSE DEHYDROGENASE (UGD), BIFUNCTIONAL ALCOHOL/UDP-GLUCOSEDEHYDROGENASE (ADH), UDP-GLUCURONATE DECARBOXYLASE (UXS), CELLULOSE SYNTHASE-LIKE D (CSLD)] and (C) xylem-specific transcripts involved in transcriptional regulation [REVOLUTA (REV), SHORT VEGETATIVE PHASE (SVP), RELATED-TO-APATELLA 2 (RAP2.12), NUCLEIC ACID BINDING/ZINC ION BINDING (RSZ33)]. Fold change relative to the respective wt is shown.