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isl2a does not contribute to endocrine cell formation. (A–C) Immunostainings for Ins (green) and Isl-proteins (red) in the pancreatic region of control (A), isl1 mutant (B), and isl1 mutant/MOisl2a injected embryos (C) at 48 hpf. (D) Quantification of ins mRNA positive cells and examples of corresponding in situ stains for ins mRNA in isl1 mutants (E, G), and in isl1 mutant, MOisl2a injected embryos (F, H) at 30 hpf (E, F) and 72 hpf (G, H). Bars show mean+SEM.
