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. Author manuscript; available in PMC: 2013 May 18.
Published in final edited form as: J Cell Biochem. 2010 Apr 15;109(6):1172–1184. doi: 10.1002/jcb.22496

Fig. 3. EtOH and PRAS40 knockdown alter mTOR and raptor phosphorylation.

Fig. 3

Scrambled control and PRAS40 knockdown cells were incubated in the presence or absence of 100 mM EtOH for 18-24 h. Cell extracts were analyzed via Western blotting using antibodies that recognize phosphorylated (P) mTOR (S2448) and raptor (S792) as well as total (T) mTOR, raptor and GβL (panel A). Panels B-D, phosphorylation levels of mTOR and raptor, as well as total GβL were quantified in bar graphs. Results were normalized to total protein and expressed as a percentage of scrambled control levels. Each bar graph represents mean ± SE of 4 independent experiments consisting of 4 replicate samples per experiment. Groups with different letters are significantly different from one another (P< 0.05). Groups with the same letters are not significantly different.

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