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. Author manuscript; available in PMC: 2013 May 18.
Published in final edited form as: J Cell Biochem. 2010 Apr 15;109(6):1172–1184. doi: 10.1002/jcb.22496

Fig. 5. Interaction of mTOR with PRAS40, raptor and GβL in EtOH and PRAS40 knockdown cells.

Fig. 5

Scrambled control and PRAS40 knockdown myocytes were incubated in the presence or absence of EtOH. mTOR was immunoprecipitated from equal amounts of cell extracts and then immunoblotted with antibodies specific for phosphorylated (P) PRAS40 (T246), raptor (S792), as well as total (T) PRAS40, raptor and GβL. Results were normalized with immunoprecipitated mTOR, which was assessed by immunoblotting. Data are mean ± SE of 4-6 independent experiments consisting of 3 replicate samples per experiment. Groups with different letters are significantly different from one another (P< 0.05). Groups with the same letters are not significantly different.

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