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. Author manuscript; available in PMC: 2013 May 18.
Published in final edited form as: J Cell Biochem. 2010 Apr 15;109(6):1172–1184. doi: 10.1002/jcb.22496

Fig. 6. Association of raptor and PRAS40 with 14-3-3 in EtOH and PRAS40 knockdown cells.

Fig. 6

Scrambled control myocytes were incubated in the presence or absence of EtOH for 18-24 h, and these were compared to PRAS40 knockdown cells. Raptor or PRAS40 were immunoprecipated from equal amounts of cell extracts and then immunoblotted with antibody against 14-3-3 θ. Levels of interaction of Raptor /14-3-3 (panel B) and PRAS40/14-3-3 (panel C) were quantified in bar graphs. Results were normalized with immunoprecipitated (IP) raptor or PRAS40. Data are mean ± SE of 3 independent experiments consisting of 3 replicate samples per experiment. * P< 0.05 versus the control values.

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