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. 2004 Feb 17;101(9):2924–2929. doi: 10.1073/pnas.0308604100

Fig. 1.

Fig. 1.

Ghrelin mRNA and protein are up-regulated in the islets of Nkx2.2 mutant mice. Immunohistochemical analysis of sectioned wild-type pancreas (a–e) or mutant pancreas (f–j) is shown. All data are from neonatal pancreata except c and h, which show pancreata from e16.5 embryos. Dashed lines delineate the pancreas at e16.6 (c and h) and an islet in neonates (d, f, and g). (a and f) Expression of amylase (green) and insulin (red). (b and g) Expression of amylase (green) and glucagon (red). (c and h) RNA in situ analysis of ghrelin mRNA in e16.5 pancreas. Incubation time for wild-type and mutant samples was identical. (d and i) Expression of ghrelin protein (red) and amylase (green). (e and j) Expression of ghrelin (red) and neuroendocrine marker chromogranin A (green).