Fig. 3.

Transcription factor profile of ghrelin-positive cells in wild-type islets. Immunofluorescence analysis of sectioned embryonic pancreas shows ghrelin (green), transcription factors, and glucagon (red). (a and b) Ghrelin expression in wild-type pancreas at e10.5. (a) Ghrelin is not coexpressed with Pdx1. (b) Ghrelin-positive, glucagon-positive cell (white arrowhead) and ghrelin-positive, glucagon-negative cell (white arrow). (c and d) e12.5: ghrelin is not expressed with Pdx1 (c) or Nkx6.1 (d). (e and f) e11.5: Pax6 is expressed in some, but not all, ghrelin cells (e). Nkx2.2 is expressed at low levels or not at all in ghrelin-positive cells (arrowhead) (f). Nkx2.2-high-expressing cells do not coexpress ghrelin (white arrowhead).