Table 2. γ-induced expression of Mdm2 and p21/WAF1/CIP1 mRNAs.
| HUF | p53 status* | Mdm2† | p21† |
|---|---|---|---|
| UV19 | WT | 5.6 ± 1.2 | 4.2 ± 0.8 |
| CO23 | WT | 5.1 ± 0.3 | 4.6 ± 1.0 |
| UV1 | WT | 4.5 ± 0.4 | 3.1 ± 0.4 |
| UV3 | WT | 3.9 ± 1.1 | 2.5 ± 0.7 |
| CO8 | WT | 3.7 ± 0.8 | 5.0 ± 0.3 |
| UV21 | Mu | 2.6 ± 0.5 | 2.6 ± 0.2 |
| UV13 | WT | 2.4 ± 0.7 | 2.1 ± 0.2 |
| UV18 | WT | 2.3 ± 0.4 | 1.9 ± 0.1 |
| CO15 | Mu | 2.2 ± 0.5 | 2.4 ± 0.3 |
| CO17 | Mu | 1.1 ± 0.1 | 1.7 ± 0.3 |
| UV5 | Mu | 1.1 ± 0.2 | 1.7 ± 0.3 |
| UV24 | Mu | 1.1 ± 0.2 | 1.3 ± 0.5 |
| UV20 | Mu | 1.0 ± 0.1 | 1.5 ± 0.1 |
| UV17 | Mu | 0.9 ± 0.1 | 1.4 ± 0.2 |
*
P53 status: WT, wild-type, no p53 DBD mutation; Mu, p53 DBD mutation (see Table 1).
†
Level of induction with SD is given as fold-increase over nonirradiated cells and normalized against GAPDH message level. Cells were collected 4 h after 20-Gy irradiation.