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Expression of Cav3, HCN4, and α-actinin in d30, d60, and d110 hESC-CM. (A) Cav3 and HCN4 double staining (d30: white arrow=low density; yellow arrow=high density) (B) α-actinin and HCN4 double staining to verify expression of HCN4 in cardiac-specific cells. Scale bar=10 μm. Color images available online at www.liebertpub.com/scd
