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Reduced fertility in ana, hma, and tms mutants. (A-C) Immature siliques from ana (A), hma (B), and tms (C) heterozygous mutants. ana produces predominantly late aborting seeds (a), rare early aborted seeds (ea), and occasionally some infertile ovules (i). hma shows ≈40% infertile ovules (arrow), and tms displays close to 25% aborted seeds (arrow). (Bar = 215 μm.) (D) Southern blot analysis of genomic DNA from heterozygous ana, hma, and tms using the Ds5′ probe (see also Fig. 6).
