Fig. 2.

Polymorphic marker analysis of mutant Ler/Col F₁ hybrids. (A) Polymorphisms at the ana locus were determined by PCR amplification by using DNA template from the mutant Ler/Col F₁ hybrid (Col × ana) and the controls Ler, Col, and Col/Ler hybrid (Col × Ler) (see Fig. 7 for hma and tms). (B) The position of each marker located on distinct BAC clones and the position of the Ds element are schematically drawn (not to scale). Bars in red indicate the deleted regions on the mutant chromosomes. Ds flanking primers were used in combination with primers at the end of Ds (Ds3-1 and Ds5-1, ref. 36) to confirm the insertion position for each mutant (Ds3′ and Ds5′).