Table 2. Cell-associated viral load and detection of vaccine and challenge forms of SIV by PCR 7-8 days after challenge.
SIVΔnef-infected monkeys
|
Control nonvaccinated monkeys
|
|||
---|---|---|---|---|
Tissue examined | 10124 | 10130 | 10674 | 10678 |
PBMC | 1(V) | — | 256 | 256 |
Palatine tonsil | 4(V/C) | 4(V) | 4,096(C) | 4,096(C) |
Spleen | 16(V) | 64(V) | 4,096 | 1,024 |
LN axillary | 32(V) | 32(V) | 128 | 1024 |
LN mesenteric | 8(V) | 8(V) | 4096 | 256 |
LN submandibular | 8(V) | 128(V) | 1,024 | 1,024 |
LN retropharyngeal | 2(C) | 16(V) | 4,096 | 1,024 |
Thymus | — | 1(V) | 128 | 2 |
Cell-associated viral load expressed as infectious units per 106 mononuclear cells. Characterization of reisolates by PCR are shown in parentheses.—, virus isolation negative; V, vaccine virus; C, challenge virus; nd, not done. In the control animals, we performed PCR on only one to two reisolates per monkey, as indicated, to verify the presence of wild type challenge virus.