Table 3. Cell-associated viral load and detection of vaccine and challenge forms of SIV by PCR 14-15 days after challenge.
| SIVΔnef-vaccinated monkeys
|
Control nonvaccinated monkeys
|
|||||||
|---|---|---|---|---|---|---|---|---|
| 10138 | 10140 | 11112 | 11118 | 10685 | 10686 | 10931 | 10932 | |
| PBMC | C | 64(nd) | — | 2(C) | 8,192 | 512 | 128(C) | 128(C) |
| Palatine tonsil | 16(V) | 4,096(V/C) | 32(V/C) | 32(V/C) | 8,192(C) | 4,096(C) | 512 | 32 |
| Spleen | 0.33(V) | 4,096(V) | 8(V) | 4(V) | 8,192 | 8,192 | 2,048(C) | 256 |
| LN axillary | 4(V/C) | 512(V/C) | 8(V) | 8(V) | 4,096 | 8,192 | 8192 | 1,024 |
| LN mesenteric | — | 1,024(V/C) | — | 4(C) | 4,096 | 8,192 | 4096 | 8,192 |
| LN submandibular | 4(V) | 1,024(V/C) | 4(V) | 32(V/C) | 1,024 | 8,192 | 256 | 512 |
| LN retropharyngeal | 0.33(V) | 1,024(V/C) | 4(C) | 16(nd) | 4,096 | 8,192 | 4096 | 512 |
| Thymus | — | 0.33(V/C) | — | 0.33(C) | NA | 64 | 1,024 | 1,024 |
Cell-associated viral load expressed as infectious units per 106 mononuclear cells. Characterization of reisolates by PCR are shown in parentheses.—, virus isolation negative; V, vaccine virus; C, challenge virus; nd, not done; NA, reisolate not available due to bacterial contamination. In the control animals, we performed PCR on only one to two reisolates per monkey, as indicated, to verify the presence of wild-type challenge virus.