Figure 2.

Detection and antigenic specificity of PAA in human sera. A. Sera (n = 54) that were tested for ICA, GADA, and IA-2A clinically by RIPA or healthy normal donor control sera (n = 10) were used to detect PAA using a standard LIPS assay (10μl serum) with Rluc-Pdx1 fusion protein lysate. RLU are expressed as fold relative to control mean. AAb = autoantibody. B. Sera (10μl) that were negative- (Ctrl), medium- (A & B), or high-signal positive (C) for PAA (from T1D patients awaiting renal transplants) were used in a standard LIPS assay using either Rluc-Pdx1 antigen or Rluc-only antigen for detection. C. A standard LIPS assay was performed to detect PAA from our highest signal positive serum (10μl) incubated with indicated concentrations of rPdx1 or BSA.