Figure 9.

GLP-1R downregulation in HIT-T15 cells. HIT-T15 cells were transfected with specific siRNA for GLP-1R (GLP-1R-siRNA) or a random sequence (scrambled-siRNA). (a) Expression of GLP-1R was analyzed 48 hours and 7 days after transfection. (b) Transfection of HIT-T15 cells with GLP-1R siRNA results in more than 50% knockdown of GLP-1R expression. (c) Five days after treatment with GS, cells were lysed and immunoblotted with specific antibodies against MafA, PDX-1, and β-actin proteins. Blots are representative of three independent experiments. *P < 0.05 and ***P < 0.001 versus scrambled siRNA.