Table 1.
DNA sequences of the sense and antisense primers for RT-PCR analysis and cycler conditions.
| Gene | Accession number (GeneBank) | Primer sequences | Product size (bp) | Annealing temperature (°C) | Number of cycles |
|---|---|---|---|---|---|
| Preproinsulin | XM_003508080.1 | 5′-CTTTTGTCAAACAGCACCTTTGTG-3′ (sense)
5′-TCAGTTGCAGTAGTTCTCCAGTTGG-3′ (antisense) |
263 | 48 | 25 |
| PDX-1 | XM_003496658.1 | 5′-CAGCTCCCTTTCCCGTGG A-3′ (sense)
5′-GAGCATCACTGCCAGCTCCA-3′ (antisense) |
204 | 55 | 28 |
| Nrf2 | XM_003498316.1 | 5′-CTCACTGGATAAAGAAGTC-3′ (sense) 5′-CCGCCCAGAAGTTCAGAGA-3′ (antisense) | 240 | 60 | 40 |
| β-Actin | NM_031144.2 | 5′-AAGAGAAGCTGTGCTATGTTGC-3′ (sense)
5′-CCTTGATCTTCATGGTGCTAGG-3′ (antisense) |
324 | 48 | 25 |
PDX-1, Pancreatic and duodenal homeobox 1; Nrf2, Nuclear factor (erythroid-derived 2)-like 2.