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. Author manuscript; available in PMC: 2013 May 19.
Published in final edited form as: Crit Care Med. 2011 Dec;39(12):2711–2721. doi: 10.1097/CCM.0b013e3182284a5f

Figure 2. Chronic amifostine preconditioning alleviates lung vascular permeability and cellular infiltration induced by high tidal volume mechanical ventilation.

Figure 2

Mice were pretreated with vehicle or amifostine (25 mg/kg) for 3 days followed by ventilation at HTV. Control animals were treated with vehicle (PBS) or amifostine alone. A - Lung vascular permeability was assessed by Evans blue accumulation in the lungs. The panels show right and left lungs respectively, which were excised after perfusion to remove blood and imaged. Bar graph depicts quantitative spectrophotomertic analysis of Evans blue-labeled albumin content in the lung tissues. Data are presented as mean ± SD; n=4-6 mice per group; ***p<0.001. B - Lung specimen from control and experimental animals were stained with hematoxylin and eosin. Images are representative of 4-6 lung specimen for each condition; ×40 magnification.