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. Author manuscript; available in PMC: 2013 May 19.
Published in final edited form as: Crit Care Med. 2011 Dec;39(12):2711–2721. doi: 10.1097/CCM.0b013e3182284a5f

Figure 7. Amifostine inhibits cyclic stretch- and thrombin-induced ROS production in human pulmonary EC.

Figure 7

Control and amifostine-preconditioned (Amif) HPAEC grown on BioFlex plates were exposed to 18% cyclic stretch (CS) followed by thrombin (Thr) stimulation or left under static conditions. To detect ROS, 30 min prior to termination of the experiment cells were incubated with fluorescent dye for ROS, dichlorodihydrofluorescein (DCFDA, 10 μM) under continuing CS stimulation. A -Cells were imaged using inverted fluorescent microscope. B - Fluorescence intensity determined in vehicle control cells was taken as 100%. Results of 6 images from the entire cell monolayer were averaged and have been reproduced in 3 independent experiments. Results are presented as mean ± SD; ***p<0.001.