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. Author manuscript; available in PMC: 2013 May 19.
Published in final edited form as: J Tissue Eng Regen Med. 2007 Nov-Dec;1(6):465–468. doi: 10.1002/term.55

Figure 1.

Figure 1

Isolation and characterization of bone marrow derived mesenchymal stem cells from L2G85 transgenic mice (MSCsFluc+/eGFP+). (a) Morphology of MSCsFluc+/eGFP+ in brightfield and fluorescence microscopy. (b) MSCsFluc+/eGFP+ show robust correlation of firefly luciferase activities and cell numbers. Bioluminescence imaging was performed on varying numbers of MSCsFluc+/eGFP+ plated on 24-well plates. (c) MSCsFluc+/eGFP+ showed 87±8% GFP positive, 94±11% CD44, 90±5% CD90, 3±0.4% CD34, and 0.1±0.05% CD45. All samples were performed in triplicates. (d) MSCsFluc+/eGFP+ morphology after culturing in the presence of different biomatrices for 48 hours. The same culture condition and same cell number was tested for all group. Scale bars: 50 μm in panel a and 100 μm in panel d.