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. Author manuscript; available in PMC: 2013 May 19.
Published in final edited form as: Circulation. 2008 Sep 30;118(14 0):S226–S233. doi: 10.1161/CIRCULATIONAHA.107.760785

Figure 1. Optimization of knocking-down target of mouse PHD2 gene.

Figure 1

(a) Individual sequences of four siRNA target sites against the PHD2 gene. (b) Schema of the tandem type shRNA structure. Two H1 promoters are used to drive the separate transcription of the sense and antisense strands. The two strands would then anneal to form a double-stranded siRNA complex within the cell. (c) Forty-eight hours after siRNA fragment transfection, comparison of knocking-down efficiency was tested by RT-PCR of mouse PHD2 gene. Data shown here indicate that the site-2 siRNA fragment had the best interference efficiency.