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. Author manuscript; available in PMC: 2013 May 19.
Published in final edited form as: Cloning Stem Cells. 2007 Winter;9(4):549–563. doi: 10.1089/clo.2007.0032

Figure 3. Expression of double fusion reporter gene in ES cells.

Figure 3

(a) Schema of lentiviral construct showing ubiquitin promoter driving Fluc and eGFP linked by a 14-amino acid linker (LENSHASAGYQAST). (b) Brightfield and fluorescence microscopy show robust eGFP expression in undifferentiated ES cells that remain stable for >3 months. (c) Bioluminescence imaging of ES cells plated on 24-well dishes shows photon signals generated by interaction of Fluc reporter protein and D-Luciferin reporter probe. (d) The strong correlation (R2=0.99) between Fluc activity and cell number serves as an in vitro validation that imaging can be used to follow cell survival and proliferation in vivo.