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. Author manuscript; available in PMC: 2013 May 19.
Published in final edited form as: Cloning Stem Cells. 2007 Winter;9(4):549–563. doi: 10.1089/clo.2007.0032

Figure 4. Reporter gene and Bi-Tet systems do not affect ES cell pluripotency.

Figure 4

(a) Brightfield and fluorescence microscopy of a representative beating embryoid body at day 14 of differentiation. (b) Immunostaining of isolated single cells confirms the expression of cardiac specific markers such as troponin-T and connexin-43. Green, GFP; Red, TRITC-antibody stain; Blue, DAPI-stained nuclei. (c) Histology of ES cells injected subcutaneously showing in vivo differentiation into striated cardiomyocytes. (d) RT-PCR comparison of Bi-Tet (−Dox) and Bi-Tet (+Dox) cells at day 0 and day 14 of differentiation. There is no significant difference in expression of ES cell (Oct4), endoderm (AFP), mesoderm (Flk-1), ectoderm (Ncam), and cardiac specific markers (MLC2v, Nkx2.5, β-MHC) between the 2 groups, suggesting that reporter gene and Bi-Tet systems do not affect ES cell pluripotency.