Skip to main content
. Author manuscript; available in PMC: 2013 May 19.
Published in final edited form as: Methods Mol Biol. 2011;750:101–114. doi: 10.1007/978-1-61779-145-1_7

Figure 3.

Figure 3

Reporter gene imaging of ES cells in living animals. (a) of Fluc positive ES cells. (a) Cell plate imaging of TF positive ES cells. 1×105, 5×105, 1×106, 2×106, 4×106, 8×106, and 1×107 mouse ES cells were plated in a 6-well plate. (b) Linear correlation of cell number and BLI signal (photons/second/cm2/steradian) reveals a strong correlation (R2=0.93). (c) Longitudinal imaging of transplanted ES cells in an immunodeficient nude rat. Transplantation of 1×107 ES cells into the myocardium leads to teratoma growth by week 4 as monitored by progressive increases in BLI and PET signals. For PET images, the animal was injected via tail vein with close to 100 μCi of the PET reporter probe [18F]-FHBG. Images were acquired 1 hour after radiotracer administration to allow for adequate biodistribution. (Reproduced from reference #4 with permission from Lippincott Williams & Wilkins)