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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Nanotoxicology. 2012 Oct 29;7:1272–1281. doi: 10.3109/17435390.2012.736547

Figure 2.

Figure 2

Cytotoxicity of MWNTs suspended in F-68, F-127 or BSA as a function of bath sonication time at 37 kHz and 120 W. After sonication, MWNT suspensions were diluted with non-sonicated F-68, F-127 or BSA solution to an equal MWNT concentration of 200 μg/mL. After dilution with an equal volume of 2X concentrated cell culture media, NRK cells were incubated for 24 h with the various MWNT suspensions at a final MWNT concentration of 100 μg/mL or in control media that contained no MWNTs. Cytotoxicity was assessed by cell proliferation, quantified by the crystal violet assay. Each data point is the mean of three independent experiments ± SD.