Figure 7.

Effect of glutathione on the cytotoxicity of F-68 after bath sonication. F-68 at a concentration of 0.2 mM was bath sonicated at 37 kHz and 120 W. NRK cells were incubated for 24 h in media containing either sonicated or non-sonicated F-68. Freshly prepared glutathione was added to the media at the beginning of the incubation. The final concentrations of F-68 and glutathione in media were 0.1 and 10.0 mM, respectively. Control cells were incubated in medium that contained no F-68 or glutathione. Cytotoxicity of sonicated F-68 in the presence or absence of glutathione was quantified using the crystal violet assay. Each data point is the mean of three independent experiments ± SD.