Fig. 1.

Linear representation of the ospC loci of WT, mutant, and complemented B. burgdorferi clones. The scale indicates the location of the ORFs and restriction sites in base pairs; the gene identification numbers are according to the database designation (40). Arrowheads below the diagram denote the positions of primers (Table 3) used in making the allelic exchange constructs for inactivation of ospC or complementation. Relevant restriction sites used for cloning or for Southern blot analysis are indicated.