Fig. 2.

Southern blot analysis of B. burgdorferi clones that are WT, mutant, or complemented at the ospC locus. Genomic DNA of clones B31-A3, ospC7, and ospC7/ospC⁺4 was separated through agarose gels, transferred to a membrane, and hybridized with a ³²P-labeled probe specific for ospC. There is a single AvrII site in cp26, a single SalI site in the integrated pGTEC-Δbla vector, and no SalI sites in cp26. AvaII cuts twice in the cp26 sequences flanking ospC and not in pGTEC-Δbla. The positions of nicked and linear cp26 and of ospC and its derivatives are indicated on the right. Migration positions of molecular size standards are indicated on the left.