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Western blot analysis of whole-cell lysates of B31-A3 WT, ospC7 mutant, and ospC7/ospC+4 complemented B. burgdorferi clones grown at pH 7 or pH 8 and probed with anti-OspC monoclonal antibody B5 (20). The arrow indicates OspC, which is produced at higher levels in WT and complemented cells grown at pH 7 compared with pH 8. Migration positions of molecular mass standards are indicated on the left.
