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. 2004 Feb 17;101(9):3142–3147. doi: 10.1073/pnas.0306845101

Fig. 4.

Fig. 4.

Immunoblot analysis of recombinant antigens and B. burgdorferi lysates with representative sera from mice inoculated by i.p./s.c. routes with B31-A3 WT, ospC7 mutant, and ospC7/ospC+4 complemented B. burgdorferi clones. Lane 1, whole-cell lysate of E. coli containing vector pBluescript SK (Stratagene) without insert; lane 2, whole-cell lysate of E. coli containing pBluescript SK with bmpA (encoding P39, which is commonly used as a marker for infection in animals) and a gene encoding P28 (an antigen often recognized by sera from infected animals) (21, 44); lane 3, whole-cell lysate of E. coli containing vector pET-29b without insert; lane 4, whole-cell lysate of E. coli containing pET-29b with ospC inserted (20); lane 5, B. burgdorferi whole-cell lysate. • indicates reaction with P39. ○ indicates reaction with OspC (His-tagged). Migration positions of molecular mass standards are indicated on the left.