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. 2004 Feb 17;101(9):3142–3147. doi: 10.1073/pnas.0306845101

Fig. 5.

Fig. 5.

(A) IFA of midgut tissues from partially fed nymphal ticks infected with B31-A3 WT, ospC7 mutant, or ospC7/ospC+4 complemented B. burgdorferi clones. Spirochetes were stained with hyperimmune rabbit anti-B. burgdorferi antiserum; binding was detected with Alexa 488-labeled anti-rabbit antibody. (B) Confocal image of IFA of salivary glands from partially fed nymphal ticks. Spirochetes were stained with hyperimmune rabbit anti-B. burgdorferi antiserum (detected with Alexa 488-labeled anti-rabbit antibody); salivary glands were stained with DRAQ5. Spirochetes of all three clones were within the salivary glands. (Scale bar, 10 μm.)