Table 2.

Lipid droplet protein expression and hepatic ceramide content of control-fed and ethanol-fed mice at four weeks

Data are reported as means ± SEM; n=5/group. P<0.05 is considered significant.

	CTRL	ETOH	P
Lipid droplet protein mRNA expression
Plin1	0.03 ± 0.01	0.02 ± 0.01	0.26
Plin2	112.0 ± 13.2	201.0 ± 21.2	0.002
Plin3	9.39 ± 1.0	9.58 ± 0.9	0.89
Hepatic ceramides (pmole/sample)
C14	1.40 ± 0.3	1.54 ± 0.3	0.72
C16	21.24 ± 2.8	18.96 ± 2.2	0.54
C18	3.90 ± 0.6	8.42 ± 1.1	0.006
C18:1	0.56 ± 0.1	0.90 ± 0.1	0.02
C20	25.04 ± 3.3	33.74 ± 3.3	0.10
C20:1	1.84 ± 0.1	2.42 ± 0.3	0.06
C22	33.42 ± 1.6	38.78 ± 2.7	0.13
C22:1	10.12 ± 0.5	13.16 ± 0.9	0.02
C24	30.10 ± 1.8	35.22 ± 2.4	0.12
C24:1	54.94 ± 3.0	74.46 ± 2.6	0.001
C26	0.12 ± 0.02	0.1 ± 0	0.35
C26:1	0.08 ± 0.04	0.16 ± 0.02	0.11

CTRL= control-fed mice; ETOH= ethanol-fed mice; Plin1= Perilipin 1; Plin2= Perilipin 2; Plin3= Perilipin 3. Expression of Plin1, 2 and 3 was normalized to 36B4 (phosphoriboprotein).