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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Int J Obes (Lond). 2013 Feb 5;37(11):1490–1498. doi: 10.1038/ijo.2013.9

Figure 4. Adiponectin transcription and mRNA stability.

Figure 4

(A) Inhibition of adiponectin gene promoter by TNF-α. The transcriptional activity of mouse adiponectin promoter (–1,300/+18) was analyzed in 3T3-L1 adipocytes in a transient transfection. After 24 h transfection, the cells were serum-starved overnight and treated with troglitazone (10 μM) and TNF-α (20 ng/ml) for 16 h. (B) Influence of TNF-α on the adiponectin mRNA stability. De novo mRNA transcription was inhibited by actinomycin D (5 μg/ml) in 3T3-L1 adipocytes. The residual mRNA of adiponectin was quantified by qRT-PCR. Values represent percentage of residual mRNA versus mRNA level at time 0. Results are means ± SEM (n=3). *, P< 0.05. ***, P< 0.001.