Fig. 3.

siRNA directed against mutant SOD1 reduces CsA-induced death in N2a cells that express human SOD1^G37R. (A) Sequences of the human SOD1 siRNAs used in these experiments (Upper) are aligned with the mouse SOD1 mRNA (Lower). The sequences of HSOD-124 and H-226 are not identical to mouse SOD1. Asterisks indicate mismatched nucleotides. (B) Transfection of SOD1^G37R N2a cells with human-specific SOD1 siRNAs increases the number of viable cells after treatment with 7 μg/ml (Middle) or 10 μg/ml (Bottom) CsA treatment, as compared to mock- or control (GFP siRNA)-transfected samples. Viable cells are detected with Calcein AM.