Fig. 2.

DHEA increases the number of BrdUrd⁺ cells in ltNSC^ctx in the presence of EGF and LIF. When EGF was withdrawn for 9 days, BrdUrd⁺ cells were not altered by the addition of LIF and DHEA (a). DHEA significantly increased the number of BrdUrd⁺ cells in the presence of EGF and LIF, although no significant effect was observed with DHEA in the absence of LIF (b). Photomicrographs represent BrdUrd immunoreactivity in cells grown in EGF (c) or EGF/LIF/DHEA (d) for 9 days. (Inset) Arrows designate BrdUrd-labeled cells at higher magnification. (Scale bar, 20 μm.)