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. 2004 Feb 18;101(9):3202–3207. doi: 10.1073/pnas.0307325101

Fig. 3.

Fig. 3.

DHEA dynamically regulates GFAP expression. (a) The numbers of GFAP+ cells treated with LIF (P < 0.05) and LIF/DHEA (P < 0.01) were significantly increased compared to that in the absence of both LIF and DHEA. (b) The percentages of double-staining with GFAP and BrdUrd antibody after 9-day culture with LIF and DHEA. Photomicrographs of cells cultured in the absence (c) and presence (d) of LIF/DHEA for 9 days show a difference in the number and intensity of GFAP+/BrdUrd+ cells. (Scale bar, 15 μm.) (e) Semiquantitative RT-PCR revealed that LIF/DHEA treatment increased GFAP mRNA compared to LIF alone. P, a positive control using total RNA isolated from human embryonic brain; N, a negative control, reverse transcriptase withdrawn during RT step. (f) Western blot analysis confirmed the effect of DHEA on GFAP protein expression.