Fig. 1.

Examples of fluorophore-assisted carbohydrate electrophoresis identifying saccharides following enzymatic digestion of theca interna isolated from both healthy and atretic follicles of different sizes (small, medium and large) with either (A) hyaluronidase SD and chondroitinase ABC or (B) heparinase and heparitinase I and II. In (A) glucose, maltose, maltotriose and maltotetraose which are not the products of hyaluronidase SD and chondroitinase ABC digestions are also visible. In each gel sacharide standards (Std) and a control without sample but still reacted with 2-aminoacridone (Blank) were included. The abbreviated names of each saccharide are listed in full in Table 1. An equivalent amount of each sample containing 3 μg of DNA was loaded per lane.